Human Anti-CD47 Recombinant Antibody (HPAB-0008-FY)
CAT#: HPAB-0008-FY
This product is a neutralizing human CD47 antibody derived from hybridomas. antibody used in various therapeutic approaches, CD47 is a ubiquitously expressed transmembrane glycoprotein with a single Ig-like domain and five transmembrane regions that serves as a cellular ligand for SIRPa through the NH2-terminal V-like structure of SIRPa Domain mediates binding.
Tested Data
Gene Expression
WB
Figure 1 Anti-Human CD47 Antibody in WB
Western blot analysis of HPAB-0008-FY was performed by loading Recombinant Human CD47 Protein (lane 1, non-reducing antigen: 1 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0008-FY and Goat Anti-Human IgG-HRP as a secondary antibody. Chemiluminescent detection was performed.
ELISA
Figure 2 Anti-Human CD47 Antibody in ELISA
ELISA analysis of HPAB-0008-FY was performed by coating with Recombinant Human CD47 Protein. Then blocked with BSA, and incubated with Anti-Human CD47 Antibody (HPAB-0008-FY). The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
SDS-PAGE
Figure 3 Anti-Human CD47 Antibody (HPAB-0008-FY) in SDS-PAGE
SDS-PAGE analysis of HPAB-0008-FY in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
HPLC
Figure 4 Anti-Human CD47 Antibody (HPAB-0008-FY) in HPLC
The purity of this antibody is> 95% as determined by SEC-HPLC.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16055/204_F3_1_selected.jpg
Subcellular Location
Figure 2 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16055/205_F3_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_A_8_3.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_A_4_6.jpg
Normal Tissue
Figure 7 Prostate
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_A_2_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000196776-CD47
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Specifications
- Immunogen
- Hu5F9-G4 recognizes human and cynomolgus CD47, but not mouse CD47.
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG
- Specificity
- Human CD47
- Species Reactivity
- Human, Cynomolgus monkey
- Applications
- ELISA, PD, Activ, PK, Stim
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CD47 Molecule; Antigenic Surface Determinant Protein OA3; CD47 Antigen (Rh-Related Antigen, Integrin-Associated Signal Transducer); Antigen Identified By Monoclonal Antibody 1D8; Leukocyte Surface Antigen CD47; Integrin Associated Protein; Rh-Related Antigen; CD47 Glycoprotein
- Gene ID
- 961
- UniProt ID
- Q08722
REVIEWS AND Q&AS
CITATIONS
RESOURCES
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User-friendly
User-friendly design and clear instructions make this antibody product easy to incorporate into our existing protocols without any hassle.
High sensitivity
The high sensitivity of this antibody product has enabled us to detect even low-abundance targets with confidence.
Reliable shipping
Shipping has always been reliable, with the product arriving in perfect condition and on time, which is crucial for our time-sensitive research.
Q&As
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What distinguishes monoclonal from polyclonal antibodies?
A: Polyclonal antibodies are mixes that can target several antigens, whereas monoclonal antibodies are homogeneous and selective to a single antigen.
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How should I treat antibodies to prevent contamination?
A: Handle antibodies with aseptic procedures and personal protective equipment to avoid contamination and assure application precision.
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Are there any additional considerations while using antibodies in experiments?
A: To ensure consistent and reproducible findings, carefully follow protocols, avoid cross-contamination, and store in proper conditions.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0008-FY, RRID: AB_3111342)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "CD47"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1122z | Mouse Anti-CD47 Recombinant Antibody (clone 23B12) | WB, FC, IHC, IP, FuncS | Mouse IgG2b |
| HPAB-1082LY | Human Anti-CD47 Recombinant Antibody (HPAB-1082LY) | ELISA | Human IgG |
| HPAB-1083LY | Human Anti-CD47 Recombinant Antibody (HPAB-1083LY) | ELISA | Human IgG |
| HPAB-1084LY | Human Anti-CD47 Recombinant Antibody (HPAB-1084LY) | ELISA | Human IgG |
| HPAB-1085LY | Human Anti-CD47 Recombinant Antibody (HPAB-1085LY) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-278LC | Human Anti-CD47 Recombinant Antibody (TAB-278LC) | Block, FuncS | Human IgG, κ |
| TAB-279LC | Human Anti-CD47 Recombinant Antibody (TAB-279LC) | Block, FuncS | Human IgG, κ |
| TAB-278LC-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (TAB-278LC-S(P)) | ELISA | Human scFv |
| TAB-278LC-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (TAB-278LC-F(E)) | ELISA | Human Fab |
| TAB-279LC-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (TAB-279LC-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-280LC | Human Anti-CD47 Recombinant Antibody (TAB-280LC) | ELISA | Humanized antibody |
| TAB-281LC | Human Anti-CD47 Recombinant Antibody (TAB-281LC) | ELISA | Humanized antibody |
| TAB-282LC | Human Anti-CD47 Recombinant Antibody (TAB-282LC) | ELISA | Humanized antibody |
| TAB-283LC | Human Anti-CD47 Recombinant Antibody (TAB-283LC) | ELISA | Humanized antibody |
| TAB-284LC | Human Anti-CD47 Recombinant Antibody (TAB-284LC) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-387CQ | Mouse Anti-CD47 Recombinant Antibody (clone CBL377) | Block, FC, CyTOF | Mouse IgG1 |
| NEUT-388CQ | Mouse Anti-CD47 Recombinant Antibody (clone MIAP410) | Block, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-389CQ | Rat Anti-CD47 Recombinant Antibody (clone miap301) | FC, FuncS, Neut, WB | Rat IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0565 | Hi-Affi™ Rabbit Anti-CD47 Recombinant Antibody (clone DS565AB) | WB, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0616-CN | Recombinant Dromedary Anti-CD47 Single Domain Antibody (HPAB-0616-CN) | ELISA | VHH |
| HPAB-AP496-YC | Recombinant Alpaca Anti-CD47 Single Domain Antibody (HPAB-AP496-YC) | FC, BL, FuncS | Alpaca VHH |
| NBD-0016-YJ-VHH | Camelid Anti-CD47 Recombinant Single Domain Antibody (NBD-0016-YJ-VHH) | ELISA, SPR | Camelid VHH |
| NBD-0017-YJ-VHH | Camelid Anti-CD47 Recombinant Single Domain Antibody (NBD-0017-YJ-VHH) | ELISA, SPR | Camelid VHH |
| NBD-0018-YJ-VHH | Camelid Anti-CD47 Recombinant Single Domain Antibody (NBD-0018-YJ-VHH) | ELISA, SPR | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1082LY-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (HPAB-1082LY-S(P)) | ELISA | Human scfv |
| HPAB-1083LY-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (HPAB-1083LY-S(P)) | ELISA | Human scfv |
| HPAB-1084LY-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (HPAB-1084LY-S(P)) | ELISA | Human scfv |
| HPAB-1085LY-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (HPAB-1085LY-S(P)) | ELISA | Human scfv |
| HPAB-1086LY-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (HPAB-1086LY-S(P)) | ELISA | Human scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1088LY-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (HPAB-1088LY-F(E)) | ELISA | Human Fab |
| HPAB-1089LY-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (HPAB-1089LY-F(E)) | ELISA | Human Fab |
| HPAB-1090LY-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (HPAB-1090LY-F(E)) | ELISA | Human Fab |
| HPAB-1091LY-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (HPAB-1091LY-F(E)) | ELISA | Human Fab |
| HPAB-1092LY-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (HPAB-1092LY-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0310-CN | Mouse Anti-CD47 Recombinant Antibody (clone 1A9) | IF, IHC, FC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY99 | Mouse Anti-NHP CD47 Recombinant Antibody (clone MEM-122) | FC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY55 | CytoStream™ Mouse Anti-CD47 Recombinant Antibody (clone B6H12) | FC | Mouse IgG1, kappa |
| VS-0225-XY56 | CytoStream™ Mouse Anti-CD47 Recombinant Antibody (clone CC2C6) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC635 | Recombinant Anti-CD47 Vesicular Antibody, EV Displayed (VS-0425-YC635) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1191 | Anti-CD47 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1192 | Anti-Human CD47 Immunohistochemistry Kit | IHC |
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